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東邦大学理学部
生物分子科学科
佐藤研究室

〒274-8510
千葉県船橋市三山2-2-1
習志野学事部入試広報課
TEL 047-472-0666

研究紹介・研究業績

 

水溶性クロロフィルタンパク質 (WSCP) の構造と機能に関する研究

水溶性クロロフィルタンパク質(water-soluble chlorophyll-binding protein: WSCP)は、今から半世紀も前に東邦大学理学部で発見された機能のよく解らないクロロフィル結合タンパク質です。WSCPは世界的にも東邦大学において先端的に研究され、当研究室ではこの研究を引き継ぎ、アブラナ科植物やヒユ科植物のWSCPの構造と機能について、分子生物学的なアプローチを行っています。
WSCPの構造と機能

緑藻オオハネモの海洋元素の高度生物濃縮能に関する研究

オオハネモは一個の細胞が大きなもので30 cmにも及ぶ多核囊状単細胞の海産緑藻類であり、海水中のさまざまな元素を高度に生物濃縮します。主に周期表第2族、第7族、第17族の元素を大量に蓄積し、特にストロンチウムは、乾燥した1 kgのオオハネモ中に16 ~ 20 gも含まれています。現在は、オオハネモが持つ海洋元素濃縮機構を、放射性ストロンチウム等を使って生理生化学的・分子生物学的な手法によって解析しています。
オオハネモ

高等植物の花弁におけるカロテノイド系色素の発現制御に関する研究

陸上植物の花の色は極めて多彩です。花弁で生合成される色素群のなかでも、バラやカーネーションなどで改変が試みられているフラボノイド系色素の研究に比較して、キクなどに見られるカロテノイド系色素の代謝に関わる遺伝子群の発現調節機構や、その人工的な改変に関する研究は多くない現状です。私たちは、花弁や葯におけるカロテノイド系色素の合成・分解に関与する一連の酵素遺伝子群の発現様式やその、人工的な改変を試みる事で花色を改変することを目指しています。
カロテノイド系色素
卒業研究テーマとしては、以上のテーマ以外にも学生の興味などで様々なテーマで研究を行っています。

主な研究業績

  Mayuko Ono, Shigekazu Takahashi, Risa Shibata, Mizuki Tomizawa, Katsumi Nakayama and Hiroyuki Satoh: Molecular Cloning and Functional Expression of A Class IIA Water-Soluble Chlorophyll-Binding Protein From Brassica nigra. J. Botanical Sci., 5:33, 2016.  

  Kyoko Aizawa, Shigekazu Takahashi, Katsumi Nakayama, and Hiroyuki Satoh: Subcellular localization of Arabidopsis thaliana heme-binding protein 1 (AtHBP1), AtHBP2 and AtHBP3J. Plant Biochem. Biotechnol. 25:3215, 2016.

  Shigekazu Takahashi, Kyoko Aizawa, Katsumi Nakayama and Hiroyuki Satoh: Water-soluble chlorophyll-binding proteins from Arabidopsis thaliana and Raphanus sativus target the endoplasmic reticulum body. BMC Res. Notes 8:365, 2015.

  Shigekazu Takahashi, Kyoko Aizawa, Saki Nakamura, Katsumi Nakayama, Shingo Fujisaki, Soichiro Watanabe, Hiroyuki Satoh: Accumulation of alkaline earth metals by the green macroalga Bryopsis maximaBioMetals Apr;28(2):391-400, 2015.

  Shigekazu Takahashi, Akira Uchida, Katsumi Nakayama, and Hiroyuki Satoh: Effect of near-infrared irradiation on photoconversion of the water-soluble chlorophyll-binding protein of Chenopodium album. Biosci. Biothech. Biochem. 79(3):439-42, 2015.

  Shigekazu Takahashi, Yumiko Seki, Akira Uchida, Katsumi Nakayama, Hiroyuki Satoh: Are tyrosine residues involved in the photoconversion of the water-soluble chlorophyll-binding protein (WSCP) of Chenopodium albumPlant Biol. 17(3),632-638, May 2015.

  Shigekazu Takahashi, Eriko Abe, Katsumi Nakayama, Hiroyuki Satoh: Identification of genes encoding photoconvertible (Class I) water-soluble chlorophyll-binding proteins from Chenopodium ficifoliumBiosci. Biothech. Biochem79(2), 205-210, 2015.

  Chieko Sugita, Yoshihiro Komura, Korechika Tanaka, Kazuki Kometani, Hiroyuki Satoh,  Mamoru Sugita: Molecular characterization of three PRORP proteins in the moss Physcomitrella patens: nuclear PRORP protein is not essential for moss viability.  PLOS ONE 2014, Oct 1;9(10):e108962, 2014

  Shigekazu Takahashi, Akira Uchida, Katsumi Nakayama,Hiroyuki Satoh: Three-step photoconversion of only three subunits of the water-soluble chlorophyll-binding protein tetramer from Chenopodium album. Protein J., 33, (4), 337-343, 2014.

  Shigekazu Takahashi, Akira Uchida, Katsumi Nakayama, Hiroyuki Satoh: The C-terminal extension peptide of non-photoconvertible water-soluble chlorophyll-binding proteins (Class II WSCPs) affects their solubility and stability: comparative analyses of the biochemical and chlorophyll-binding properties of recombinant Brassica, Raphanus and Lepidium WSCPs with or without their C-terminal extension peptides. Protein J., 33,75-84,2014

  Shigekazu Takahashi, Haruna Yanai, Yuko Oka-Takayama, Aya Zanma-Sohtome, Kosaku Fujiyama, Akira Uchida, Katsumi Nakayama, Hiroyuki Satoh: Molecular cloning, characterization and analysis of the intracellular localization of a water-soluble chlorophyll-binding protein (WSCP) from Virginia pepperweed (Lepidium virginicum), a unique WSCP that preferentially binds chlorophyll b in vitro Planta, 238(6),1065-1080, 2013

  Shigekazu Takahashi, Mami Yoshikawa, Akiko Kamada, Takayuki Ohtsuki, Akira Uchida, Katsumi Nakayama, & Hiroyuki Satoh: The photoconvertible water-soluble chlorophyll-binding protein of Chenopodium album is a member of DUF538, a superfamily that distributes in Embryophyta. J. Plant Physiol., 170, 1549-1552, 2013

  Ayano Imai, Shigekazu Takahashi, Katsumi Nakayama & Hiroyuki Satoh: A promoter of carotenoid cleavage dioxygenase 4a-5 gene of Chrysanthemum morifolium (CmCCD4a-5) drives a petal-specific transcription of conjugated gene in the developing flower. J. Plant Physiol., 170(14), 1295-1229, 2013 

  Shigekazu Takahashi, Mayuko Ono, Akira Uchida, Katsumi Nakayama & Hiroyuki Satoh: Molecular cloning and functional expression of a water-soluble chlorophyll-binding protein from Japanese wild radish. J. Plant Physiol., 170(4), 406-412, 2013

  Shigekazu Takahashi, Haruna Yanai, Yurino Nakamaru, Akira Uchida, Katsumi Nakayama, Hiroyuki Satoh: Molecular cloning, characterization and analysis of the intracellular localization of a water-soluble Chl-binding protein from Brussels sprouts (Brassica oleracea var. gemmifera). Plant Cell Physiol. 53(5), 879-891, 2012

  Marie Desclos, Lucie Dubusset, Philippe Etienne, Francoise Le Caherec, Hiroyuki Satoh, Josette Bonnefoy, Alain Ourry, & Jean-Christophe Avice: A Proteomic profiling approach to reveal a novel role of BnD22 (Brassica napus drought 22 kD)/water-soluble chlorophyll binding protein in young leaves during nitrogen remobilization induced by Stressful conditions. Plant Physiology, 147, 1830-1844, 2008

  Daisuke Horigome, Hiroyuki Satoh, Nobue Itoh, K. Mitsunaga, Isao Oonishi, Atsushi Nakagawa & Akira Uchida: Structural mechanism and photoprotective function of water-soluble chlorophyll-binding protein. J. Biol. Chem., 282(9),  6525-6531, 2007

  Hiroki Hirabayashi, M. Amakawa, Yasumaro Kamimura, Yayoi Shino, Hiroyuki Satoh, Shigeru Itoh, Hitoshi Tamiaki: Analysis of photooxidized pigments in water-soluble chlorophyll protein complex isolated from Chenopodium album. J. Photochem. Photobiol. A: Chem., 183, 121-125, 2006

  Christiane Reinbothe, Hiroyuki Satoh, Jean-Pierre Alcaraz, & Steffen Reinbothe: A novel role of water-soluble chlorophyll proteins in the transitory storage of chorophyllide. Plant Physiology, 134, 1355-1365, 2004

  Daisuke Horigome, Hiroyuki Satoh & Akira Uchida: Purification, crystallization and preliminary X-ray analysis of a water-soluble chlorophyll protein from Brassica oleracea L. var. acephala (kale). Acta Crystallographica Section D, D59, 2283-2285, 2003

  Kristin Schmidt, Christian Fufezan, Anja Krieger-Liszkay, Hiroyuki Satoh, Harald Paulsen: Recombinant water-soluble chlorophyll protein from Brassica oleracea var. botrys bind various chlorophyll derivatives. Biochemistry, 42, 7427-7433, 2003

  Hiroyuki Satoh, Aya Zanma, & Kiyoaki Shinashi: Molecular cloning and sequence analysis of a water-soluble chlorophyll protein cDNA from a Japanese radish. J. Plant Physiol., 159(3), 325-327, 2002

  Kiyoaki Shinashi, Hiroyuki Satoh, Akira Uchida, Katsumi Nakayama, Mitsumasa Okada, Isao Oonishi: Molecular characterization of a water-soluble chlorophyll protein from main veins of Japanese radish. J. Plant Physiol., 157, 255-262, 2000

  Hiroyuki Satoh, Naomi Inokuchi, Ysuhiro Nagae, Taro Okazaki: Organization, structure and evolution of rat non-adult β-globin gene cluster. J. Mol. Evol., 49(1), 122-129, 1999

  Hiroyuki Satoh, Naomi Inokuchi, Yasuhiro Nagae, Taro Okazaki: Molecular cloning and characterization of two sets of α-θ genes in the rat α-like globin gene cluster. Gene, 230, 91-99, 1999.

  Hiroyuki Satoh, Katsumi Nakayama & Mitsumasa Okada: Molecular cloning and functional expression of a water-soluble chlorophyll-protein, a putative carrier of chlorophyll molecules in cauliflower. J. Biol. Chem., 273(46) 30568-30575, 1998

  Naomi Nishio, Hiroyuki Satoh: A Water-soluble chlorophyll protein in cauliflower may be identical to BnD22, a drought-induced 22 kD-protein in rapeseed. Plant Physiology, 115(2), 841-846, 1997.

  Stephan Immenschuh, Shin-ichiro Iwahara, Hiroyuki Satoh, Christina Nell, Norbert Katz, Ursula Muller-Eberhard:  Expression of the mRNA of heme-binding protein 23 is coordinated with that of heme oxygenase-1 by heme and heavy metals in primary rat hepatocytes and hepatoma cells. Biochemistry, 34(41), 13407-13411, 1995.

  Shin-ichiro Iwahara, Hiroyuki Satoh, De-Xiu Song, James Webb, Alma L.Burlingame, Yasuhiro Nagae, Ursula Muller-Eberhard: Purification, characterization, and cloning of a heme-binding protein (23 kDa) in rat liver cytosol. Biochemistry, 34(41), 13398-13406, 1995.

  Hiroyuki Satoh, Yasuhiro Nagae, Stephan Immenschuh, Tomoko Satoh & Ursula Muller-Eberhard: Identification and characterization of a cis-acting element that suppresses an activity of the liver preference enhancer element of the rat hemopexin gene. Porphyrins, 4(1), 9-14, 1995.

  Stephan Immenschuh, De-Xiu Song, Hiroyuki Satoh & Ursula Muller-Eberhard: The type II hemopexin interleukin-6 response element predominates the transcriptional regulation of the hemopexin acute phase responsiveness. Biochem. Biophys. Res. Commun., 207, 202-208, 1995.

  Tomoko Satoh, Hiroyuki Satoh, Shin-ichiro Iwahara, Zbynek Hrkal, David H. Peyton, Ursula Muller-Eberhard: Roles of heme iron-coordinating histidine residues of human hemopexin expressed in baculovirus-infected insect cells. Proc. Natl. Acad. Sci. USA., 91, 8423-8427, 1994.

  Stephan Immenschuh, Yasuhiro Nagae, Hiroyuki Satoh, Heinz Baumann, Ursula Muller-Eberhard: The rat and human hemopexin genes contains an identical interleukin-6 response element that is not a target of CAAT enhancer-binding protein isoforms. J. Biol. Chem., 269, 12654-12661, 1994.

  Hiroyuki Satoh, Yasuhiro Nagae, Stephan Immenschuh, Tomoko Satoh, Ursula Muller-Eberhard: Identification of a liver preference enhancer element of the rat hemopexin gene and its interaction with nuclear factors. J. Biol. Chem., 269, 6851-6859, 1994.

  Midori Kono, Hiroyuki Satoh, Yasuyuki Okabe, Yasuko Abe, Katsumi Nakayama, Mitsumasa Okada: Nucleotide sequence of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from the green alga Bryopsis maxima. Plant Mol. Biol., 17, 505-508, 1991.

  Mitsumasa Okada, Yasuyuki Okabe, Midori Kono, Katsumi Nakayama & Hiroyuki Satoh: Peptide composition and enzyme activities of isolated pyrenoids from the green alga Bryopsis maxima. Can. J. Bot. 69, 1053-1061, 1990.

  Hiroyuki Satoh, Hiroyuki Fujii, Taro Okazaki: Molecular cloning and sequence analysis of two rat major globin cDNAs. Biochem. Biophys. Res. Commun. 146, 618-624, 1987.

  Mitsumasa Okada, Michiko Ohtomi, Katsumi Nakayama, Yohko Sakamoto, Hiroyuki Satoh, Masako Konagaya, Makoto Yoshizaki: Purification and characterization of a new pigment from the green alga, Bryopsis maxima. Plant Cell Physiol. 28, 1435-1441, 1987.

  Hiroyuki Satoh, Mitsumasa Okada, Katsumi Nakayama, Teruyo Murata: Purification of ribulose 5-phosphate kinase and minor polypeptides of pyrenoid from the green alga Bryopsis maxima. Plant Cell Physiol. 26, 931-940, 1985.

  Hiroyuki Satoh, Mitsumasa Okada, Katsumi Nakayama, Kazuyuki Miyaji: Purification and further characterization of pyrenoid proteins and ribulose-1,5-bisphosphate carboxylase-oxygenase from the green alga Bryopsis maxima. Plant Cell Physiol., 25, 1205-1214, 1984.