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東邦大学
薬学部 微生物学教室

〒274-8510
千葉県船橋市三山2-2-1
習志野学事部入試広報課
TEL 047-472-0666

研究業績

 

原著論文など　（2003年～）

  1. Zeng, J., Iizaka, Y., Ouchi, Y., Otsuki, K., Kikuchi, T., Li, W., Anzai, Y.. Inhibitory effects of reumycin produced by Streptomyces sp. TPMA0082 on virulence factors of Pseudomonas aeruginosa. Journal of Natural Medicines. 79(4):608-620 (2025).
  2. Asano, R., Iizaka, Y., Kashima, M., Anzai, Y., Yamaguchi, S., Tada, M. Unveiling Dynamic Hepatocyte Plasticity in HepaRG Cells with a Dual CYP Reporter System. PLOS ONE. 19(11):e0308694, (2024) 
  3. Iizaka, Y., Yamada, M., Koshino, S., Takahashi, S., Saito, R., Sherman, D. H., Anzai, Y. Production of hybrid macrolide antibiotics by exploiting the specific substrate recognition characteristics of multifunctional cytochrome P450 enzyme MycG. FEMS Microbiology Letters, 371,fnae080 (2024).
  4. 木下雅子，山岸莉奈，飯坂洋平，瀧川正紀，安齊洋次郎，浦野敦，廣瀬香織，花輪剛久，田中博之　異なる方法で調製したブロー氏液及びネオ・ブロー氏液の安定性と抗菌活性の評価　YAKUGAKU ZASSHI. 144(9):887-896 (2024)
  5. Zeng, J., Iizaka, Y., Hamada, M., Iwai, A., Takeuchi, R., Fukumoto, A., Tamura, T., Anzai, Y. Actinoplanes kirromycinicus sp. nov., isolated from soil. The Journal of Antibiotics. 77(10):657-64 (2024).
  6. Tanaka, Y., Nagano, H., Okano, M., Kishimoto, T., Tatsukawa, A., Kunitake, H., Fukumoto, A., Anzai, Y., Arakawa, K. Isolation of hydrazide-alkenes with different amino acid origins from an azoxy-alkene-producing mutant of Streptomyces rochei 7434AN4. Journal of Natural Products. 86(9):2185-92 (2023).
  7. Iizaka, Y. Study on multistep and multifunctional cytochrome P450 enzymes from actinomycetes. ACTINOMYCETOLOGICA. 37(1):32 -42 (2023).
  8. Davaapurev, B.-O., Fukumoto, A., Bolortuya, B., Boldbaatar, D., Maehata, Y., Iizaka, Y., Kato, F., Batkhuu, J., Anzai, Y. Methyl-β-D-glucopyranoside from Scabiosa comosa as a Quorum Sensing Inhibitor. Natural Product Communications. 17(11):1-4 (2022).
  9. Tatsukawa, A., Tanaka, Y. Nagano, H., Fukumoto, A., Anzai, Y., Arakawa, K. Isolation, biosynthetic investigation, and biological evaluation of maniwamycin G, an azoxyalkene compound from Streptomyces sp. TOHO-M025. Journal of Natural Products. 85(7):1867-71 (2022).
  10. Oyunbileg, N., Davaapurev, B.-O., Tsetseg, B., Iizaka, Y., Fukumoto, A., Kato, F., Anzai, Y., Batkhuu, J. Bioactive compounds and molecular diversity of endophytic actinobacteria isolated from desert plants. IOP Conference Series: Earth and Environmental Science. (908):012008 (2021).
  11. Iizaka, Y., Arai, R., Takahashi, A., Ito, M., Sakai, M., Fukumoto, A., Sherman, D. H., Anzai, Y. Engineering sequence and selectivity of late-stage C-H oxidation in the MycG iterative cytochrome P450. Journal of Industrial Microbiology and Biotechnology. 49(1):kuab069 (2021).
  12. Breiner-Goldstein, E., Eyal, Z., Matzov, D., Halfon, Y., Cimicata, G., Baum, M., Rokney, A., Ezernitchi, A.V., Lowell, A.N., Schmidt, J.J., Rozenberg, H., Zimmerman, E., Bashan, A., Valinsky, L., Anzai, Y., Sherman, D.H., Yonath, A. Ribosome-binding and anti-microbial studies of the mycinamicins, 16-membered macrolide antibiotics from Micromonospora griseorubida. Nucleic Acids Research. 49(16):9560-73 (2021).
  13. Oyunbileg, N., Iizaka, Y., Hamada, M., Davaapurev, B.-O., Fukumoto, A., Tsetseg, B., Kato, F., Tamura, T., Batkhuu, J., Anzai, Y. 11. Actinocatenispora comari sp. nov., an endophytic actinomycete isolated from aerial parts of Comarum salesowianum. International Journal of Systematic and Evolutionary Microbiology.
    71(7): ijsem.0.004861 (2021).
  14. Iizaka, Y., Sherman, D.H., Anzai, Y. An overview of the cytochrome P450 enzymes that catalyze the same-site multistep oxidation reactions in biotechnologically relevant selected actinomycete strains. Applied Microbiology and Biotechnology. 105(7):2647–2661 (2021).
  15. Yang, S., DeMars II, M.D., Grandner, J.M., Olson, N.M., Anzai, Y., Sherman, D.H. Houk K.N. Computational-based mechanistic study and engineering of cytochrome P450 MycG for selective oxidation of 16-membered macrolide antibiotics. Journal of the American Chemical Society. 142(42):17981−8 (2020).
  16. Iizaka, Y., Kanai, H., Suzuki, T., Maruyama, Y., Kurita, M., Sano, M., Watanabe, A., Fukumoto, A., Saito, R., Anzai, Y. Artificial control of the multistep oxidation reactions catalyzed by the cytochrome P450 enzyme RosC.  Applied Microbiology and Biotechnology. 104(8):3403-15 (2020).
  17. Ohta, T., Fukumoto, A., Iizaka, Y., Kato, F., Koyama, Y., Anzai, Y. Quorum sensing inhibitors against Chromobacterium violaceum CV026 derived from an actinomycete metabolite library.  Biological and Pharmaceutical Bulletin. 43(1):179-83. (2020).
  18. Benito, P., Alonso-Vega, P., Aguado, C., Luján, R., Anzai, Y., Hirsch, A.M., Trujillo, M.E. Monitoring the colonization and infection of legume nodules by Micromonospora in co-inoculation experiments with rhizobia. Scientific Reports. 7:11051 (2017).
  19. Iizaka, Y., Takeda, R., Senzaki, Y., Fukumoto, A., Anzai, Y. Cytochrome P450 enzyme RosC catalyzes a multistep oxidation reaction to form the non-active compound 20-carboxyrosamicin. FEMS Microbiology Letters. 364(12):fnx110 (2017).
  20. Fukumoto, A., Murakami, C., Anzai, Y., Kato, F. Maniwamycins: new quorum-sensing inhibitors against Chromobacterium violaceum CV026 were isolated from Streptomyces sp. TOHO-M025. The Journal of Antibiotics. 69:395-9 (2016).
  21. Bernard, S.M., Akey, D.L., Tripathi, A., Park, S.R., Konwerski, J.R., Anzai, Y., Li, S., Kato, F., Sherman, D.H., Smith, J.L. Structural basis of substrate specificity and regiochemistry in the MycF/TylF family of sugar O-methyltransferases. ACS Chemical Biology. 10(5):1340-51 (2015).
  22. 安齊洋次郎　放線菌のセシウム蓄積に関する研究　IFO Research Communications. 28:136-7 (2014). in Japanese
  23. Iizaka, Y., Higashi, N., Li, W., Fukumoto, A., Anzai, Y., Kato, F. A new mycinosyl rosamicin derivative produced by an engineered Micromonospora rosaria mutant with a cytochrome P450 gene disruption introducing the D-mycinose biosynthetic gene. Journal of Industrial Microbiology and Biotechnology. 41(9):1451-6 (2014).
  24. Zhang, W., Liu, Y., Yan, J., Cao, S., Bai, F., Yang, Y., Huang, S., Yao, L., Anzai, Y., Kato, F., Podust, L.M., Sherman, D.H., Li, S. New reactions and products resulting from alternative interactions between the P450 enzyme and redox partners. Journal of the American Chemical Society. 136(9):3640-46 (2014).
  25. Bai, H., Li, W., Zhao, H., Anzai, Y., Li, H., Guo, H., Kato, F., Koike, K.Isolation and structural elucidation of novel cholestane glycosides and spirostane saponins from Polygonatum odoratum. Steroids. 80(1):7-14 (2014).
  26. Ooka, K., Fukumoto, A., Yamanaka, T., Shimada, Y., Ishihara, R., Anzai, Y., Kato, F. Piericidins, novel quorum-sensing inhibitors against Chromobacterium violaceum CV026, from Streptomyces sp. TOHO-Y209 and TOHO-O348. Open Journal of Medicinal Chemistry. 3(4):93-9 (2013).
  27. Kobayashi, H., Isozaki, M., Fukuda, T., Anzai, Y., Kato, F. Surveillance of fluoroquinolone-resistant clinical isolates of Pseudomonas aeruginosa. Open Journal of Medical Microbiology. 3(2): 144-50 (2013).
  28. Iizaka, Y., Higashi, N., Ishida, M., Oiwa, R., Ichikawa, Y., Takeda, M., Anzai, Y., Kato, F. Function of cytochrome P450 enzymes RosC and RosD in the biosynthesis of rosamicin macrolide antibiotic produced by Micromonospora rosaria. Antimicrobial Agents and Chemotherapy. 57(3):1529-31 (2013).
  29. Li, S., Tietz, D.R., Rutaganira, F.U., Kells, P.M., Anzai, Y., Kato, F., Pochapsky, T.C., Sherman, D.H., Podust, L.M. Substrate recognition by the multifunctional cytochrome P450 MycG in mycinamicin hydroxylation and epoxidation reactions. The Journal of Biological Chemistry. 287(45):37880-90 (2012).
  30. Sakai, A., Mitsumori, A., Furukawa, M., Kinoshita, K., Anzai, Y., Kato, F. Production of a hybrid 16-membered macrolide antibiotic by genetic engineering of Micromonospora sp. TPMA0041. Journal of Industrial Microbiology and Biotechnology. 39(11):1693–701 (2012).
  31. Anzai, Y., Tsukada, S., Sakai, A., Masuda, R., Harada, C., Domeki, A., Li, S., Kinoshita, K., Sherman, D.H., Kato, F. Function of the cytochrome P450 enzymes MycCI and MycG in Micromonospora griseorubida, a producer of the macrolide antibiotic mycinamicin. Antimicrobial Agents and Chemotherapy. 56(7):3648-56 (2012).
  32. Akey, D.L., Li, S., Konwerski, J.R., Confer, L.A., Bernard, S.M., Anzai, Y., Kato, F., Sherman, D.H., Smith, J.L. A new structural form in the SAM/metal-dependent O-methyltransferase family: MycE from the mycinamycin biosynthetic pathway. Journal of Molecular Biology. 413(2):438-50 (2011).
  33. Carlson, J.C., Li, S., Gunatilleke, S.S., Anzai, Y., Burr, D.A., Podust, L.M., Sherman, D.H. Tirandamycin biosynthesis is mediated by co-dependent oxidative enzymes. Nature Chemistry. 3(8):628-33 (2011).
  34. Kuwahara, C., Fukumoto, A., Nishina, M., Sugiyama, H., Anzai, Y., Kato, F. Characteristics of cesium accumulation in the filamentous soil bacterium Streptomyces sp. K202. Journal of Environmental Radioactivity. 102(2):138-44 (2011).
  35. Anzai, Y., Sakai, A., Li, W., Iizaka, Y., Koike, K., Kinoshita, K., Kato, F. Isolation and characterization of 23-O-mycinosyl-20-dihydro-rosamicin: a new rosamicin analogue derived from engineered Micromonospora rosaria. The Journal of Antibiotics. 63(6):325-28 (2010).
  36. Tsukada, S., Anzai, Y., Li, S., Kinoshita, K., Sherman D.H., Kato, F. Gene targeting for O-methyltransferase genes, mycE and mycF, on the chromosome of Micromonospora griseorubida producing mycinamicin with disruption cassette containing bacteriophage ϕC31 attB attachment site. FEMS Microbiology Letters. 304(2):148-56 (2010).
  37. Carlson, J.C., Fortman, J.L., Anzai, Y., Li, S., Burr, D.A., Sherman, D.H. Identification of the tirandamycin biosynthetic gene cluster from Streptomyces sp. 307-9. ChemBioChem. 11(4):564-72 (2010).
  38. Anzai, Y., Iizaka, Y., Li, W., Idemoto, N., Tsukada, S., Koike, K.,Kinoshita, K., Kato, F. Production of rosamicin derivatives in Micromonospora rosaria by introduction of D-mycinose biosynthetic gene with ϕC31-derived integration vector pSET152. Journal of Industrial Microbiology and Biotechnology. 36(8):1013-21 (2009).
  39. Li, S., Anzai, Y., Kinoshita, K., Kato, F., Sherman, D.H. Functional analysis of MycE and MycF, two O-methyltransferases involved in biosynthesis of mycinamicin macrolide antibiotics. ChemBioChem. 10(8):1297-301 (2009).
  40. Sugiyama, H., Takahashi, M., Terada, H., Kuwahara, C., Maeda, C., Anzai, Y., Kato, F. Accumulation and localization of cesium in edible mushroom (Pleurotus ostreatus) mycelia. Journal of Agricultural and Food Chemistry. 56(20):9641-46 (2008).
  41. Anzai, Y., Li, S., Chaulagain, M.R., Kinoshita, K., Kato, F., Montgomery, J., Sherman, D.H. Functional analysis of MycCI and MycG, cytochrome P450 enzymes involved in biosynthesis of mycinamicin macrolide antibiotics. Chemistry and Biology. 15(9):950–59 (2008).
  42. Anzai, Y., Saito, S., Fujimoto, K., Kinoshita, K., Kato, F. Detection and identification of species with bacterial cells using a plastic DNA array. Journal of Health Science. 54(2):229-34 (2008).
  43. Tsuji, K., Asakawa,M., Anzai, Y., Sumino, T., Harada, KI. Degradation of microcystins using immobilized microorganism isolated in a eutrophic lake. Chemosphere. 65(1):117–24 (2006).
  44. Kajiura, T., Wada, H., Ito, K., Anzai, Y., Kato, F. Conjugative plasmid transfer in the biofilm formed by Enterococcus faecalis. Journal of Health Science. 52(4):358-67 (2006).
  45. Lee, K.-B., Liu, C.-T., Anzai, Y., Kim, H., Aono, T., Oyaizu, H. The hierarchical system of the 'Alphaproteobacteria': description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. International Journal of Systematic and Evolutionary Microbiology. 55(5):1907-19 (2005).
  46. Anzai, Y., Kinoshita, K., Seki, T., Kato, F. Hybrid biosynthesis by targeted inactivation of polyketide synthases in the mycinamicin producer, Micromonospora griseorubida. The Journal of Antibiotics. 57(12):819-22 (2004).
  47. Anzai, Y., Ishii, Y., Yoda, Y., Kinoshita, K., Kato, F. The targeted inactivation of polyketide synthase mycAV in the mycinamicin producer, Micromonospora griseorubida, and a complementation study. FEMS Microbiology Letters. 238(2):315-20 (2004).
  48. Kuwahara, C., Fukumoto, A., Ohsone, A., Furuya, N., Shibata, H., Sugiyama, H., Kato, F. Accumulation of radiocesium in wild mushrooms collected from a Japanese forest and cesium uptake by microorganisms isolated from the mushroom-growing soils. Science of The Total Environment. 345(1-3):165-73 (2004).
  49. Anzai, Y., Saito, N., Tanaka, M., Kinoshita, K., Koyama, Y., Kato, F. Organization of the biosynthetic gene cluster for the polyketide macrolide mycinamicin in Micromonospora griseorubida. FEMS Microbiology Letters. 218(1):135-41 (2003). 

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東邦大学薬学部微生物学教室

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