English TOP > Staff


Laboratory of Neuroscience
Department of Biology,
Faculty of Science,
Toho University

2-2-1 Miyama, Funabashi,
Chiba, 274-8510 Japan



Yoshinori MASUO, Professor

Laboratory of Neuroscience
Department of Biology, Faculty of Science / Graduate School of Science
Office: 1406A, 4th floor, 1st Bld. of Faculty of Science
Birth: Yanagawa, Fukuoka
Growth: Ohizumi, Nerima-ku, Tokyo


Year, Degree, College or University, Country, Field of Study, Supervisor
1983, Bachelor of Science, Department of Biology, Faculty of Science, Toho University, Japan, Biology, M. Toru (Neurological Research Center, present National Institute of Neuroscience)
1986, Master of Medical Science, Graduate School of Medical Science, University of Tsukuba, Japan, Neuropharmacology and Neurochemistry, I. Kanazawa
1990, Ph.D. in Neuroscience, University of Paris 6, France, Neuroscience, J. Glowinski, W. Rostène
1994, Ph.D. in Medicine, Graduate School of Medicine, University of Tokyo, Japan, Neuroscience, T. Haga

Positions held

Period (dd/mm/yy), Institution, Position held, Discipline
01/10/1990, Tsukuba Research Laboratories, Takeda Chemical Industries, Researcher, Neuroscience
01/10/1993, Discovery Research Laboratories I, Takeda Chemical Industries, Associate Research Head, Neuroscience
01/04/1997, Discovery Research Laboratories I, Takeda Chemical Industries, Research Head, Neuroscience
01/10/1997, Department of Physiology, Toho University School of Medicine, Research Associate, Neurophysiology
01/07/2000, National Institue of Bioscience and Human Technology, Researcher, Neuroscience
01/04/2001, International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology (AIST), Chief Assistant, Neuroscience
01/04/2001, New Energy and Industrial Technology Development Organization, Researcher class A, Neuroscience
01/04/2004, Stress Response Team, Human Stress Signal Research Center, National Institute of AIST, Researcher, Neuroscience
01/01/2005, Stress Response Team, Human Stress Signal Research Center, National Institute of AIST, Senior Research Scientist, Neuroscience
01/04/2005, Mental Stress Team, Human Stress Signal Research Center, National Institute of AIST, Team Leader, Neuroscience
13/02/2006, University Louis Pasteur, Invited Professor, Neuroscience
01/04/2008, Mental Stress Team, Health Technology Research Center, National Institute of AIST, Team Leader, Neuroscience
01/04/2010, Laboratory of Neuroscience, Department of Biology, Faculty of Science / Graduate School of Science, Toho University, Professor, Neuroscience

Affiliation society

1.      International Brain Research Organization (IBRO)
2.      Society for Neuroscience
3.      New York Academy of Sciences
4.      Japan Neuroscience Society
5.      Japanese Society for Neurochemistry
6.      Physiological Society of Japan
7.      Japan Society of Endocrine Disrupters Research
8.      Japanese Tissue Culture Association (member of Cell Bank committee, 2002-2004)

Learned technology

1.      Lesioning technique in a brain region with a stereotaxic apparatus
2.      Microinjection technique into a brain region and intraventricular administration
3.      Microdissection of the brain
4.      Receptor binding assay
5.      Quantitative analysis of aminoacids by fluorescent method (glutamate, aspartic acid, γ-aminobutyric acid)
6.      Measurement of enzyme activity by radiochemical measuring method (tyrosine hydroxylase, choline acetyltransferase)
7.      Quantitative analyses of amino acids, monoamines, acetylcholine, and its metabolites with high-performance liquid chromatography with electrochemical detector (HPLC-ECD)
8.      Separation of peptides from the brain with high-performance liquid chromatography (HPLC)
9.      Quantitative analyses of peptides by radioimmunoassay (substance P, neurokinin A and B, neurotensin, vasoactive intestinal contractor (VIC), etc.)
10.   Quantitative analysis of pituitary adenylate cyclase-activating polypeptide (PACAP) by sandwich enzyme immunoassay (EIA)
11.   Refining of tyrosine and separation of dopamine
12.   Measurement of the release of neurotransmitters by push-pull cannulae
13.   Measurement of the release of catecolamins and acetylcholine by microdialysis method
14.   Measurement of receptor density by quantitive autoradiography: neurotensin receptor, iodosulpride binding site (dopamine D2 receptor), dihydrotetrabenazine binding site (synaptic vesicle containing monoamine transporter), vasoactive intestinal polypeptide (VIP) receptor, PACAP receptor
15.   Measurement of gene expression of endothelin 1, VIC/, endothelin 2, and GAPDH.with quantitive real-time reverse transcription-linked polymerase chain reaction (RT-PCR) method
16.   'In situ' hybridization method using RNA (extraction of total RNA, design of primer, RT-PCR, ligation, culture of E.Coli, and refining of plasmid DNA, labeling with DIG, and hybridization)
17.   'In situ' hybridization method using oligonucleotide probe (VIP, PACAP)
18.   Immunohistochemistry (VIP, PACAP, various calcium-binding proteins, synaptophysin, microtubule-associated protein 2 (MAP2), drebrin)
19.   Determination of apoptotic cells by terminal deoxynucleotidyltransferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end labeling (TUNEL) method
20.   Electrophysiological amnalyses in the mouse hippocampal slice: measurement of field excitatory postsynaptic potential (EPSP)
21.   Behavioral analyses in the rat and mouse: motor coordination, locomotor activity, rearing, fear, lerning and memory
22.   Measurement of convulsant-threshold by electroshock method in the rat
Others: Reading and writing, conversation in Japanese and English, and conversation in French

Possible lectures

1.      Morphology and function of the central nervous system, especially on the frontal cortex, basal ganglia and hippocampus
2.      Control of motor activity: function of the basal ganglia
3.      Neurodegenerative disorders: Parkinson’s disease and Huntington’s disease
4.      Psychiatric disorders: developmental disorders and depression
5.      Stress and the brain
6.      Stress-responsive biomarkers in the brain and blood


Current members

   WAKAMATSU Hisanori (Postdoctoral fellow)
   SUZUKI Keisuke (M2)
   TOKITA Mika (M2)
   TSUNEISHI Wako (M1)
   IRIE Mai (B4)
   EMOTO Mai (B4)
   OKADA Narumi (B4)
   KISHI Satomi (B4)
   KUBOHARA Mika (B4)
   NISHII Atsuo (B4)
   MABUCHI Akifumi (B4)
   MIZUTA Keisuke (B4)

Past members

Graduated in 2013
   FUKE Rikiya (B.Sci)
   HOTTA Ryotaro (B.Sci)
   OKAMOTO Akari (B.Sci)
   SANO Fumika (B.Sci)
   TAKAURA MAsahiko (B.Sci)
   TSUNEISHI Wako (B.Sci)
Graduated in 2012
   HASHIMOTO Ohsuke (B.Sci)
   HATA Erika (B.Sci)
   MASAKI Tatsuyuki (B.Sci)
   NAGAYOSHI Saki (B.Sci)
   NAKAO Maiko (B.Sci)
   ODA Masumi (B.Sci)
   SUZUKI Keisuke (B.Sci)
   YOSHINO Akiharu (B. Sci)